Integration of Neuraminidase Inhibitor Assay into Single Step Operation Using Combinable Pdms Capillary (cpc) Sensor
نویسندگان
چکیده
Neuraminidase inhibitor assay requiring complicated step-by-step operation was successfully integrated into ”Single-Step” by using CPC sensor. We developed a combinable PDMS capillary (CPC) sensor for enzyme inhibition assay using the concave and convex type PDMS substrates. In the conventional enzyme inhibitor assay, complicated analytical procedures, such as mixing and dissolution, are required. In contrast, this sensor allows single-step assay possible only by sample introduction via capillary action. Here, we applied this technique for neuraminidase inhibitor assay as application and found appropriate experimental condition allowing single-step assay of neuraminidase inhibitor.
منابع مشابه
Single-step and Multiple Bioassay Based on Combinable Pdms Capillary (cpc) Sensor Array
Combinable poly(dimethyl siloxane) (PDMS) capillary (CPC) sensor array allowing single step and multiple bioanalysis by simply introducing a sample solution by capillary action, followed by spontaneous mixing and reactions for fluorescence readout was developed. Since the CPC is fabricated by combining "Convex"-shaped PDMS plate immobilizing an insoluble membrane containing analytical reagents ...
متن کاملPower-free sequential injection for microchip immunoassay toward point-of-care testing.
This paper presents a simple fluid handling technique for microchip immunoassay. Necessary solutions were sequentially injected into a microchannel by air-evacuated poly(dimethylsiloxane), and were passively regulated by capillary force at the inlet opening. For heterogeneous immunoassay, microchips are potentially useful for reduction of sample consumption and assay time. However, most of the ...
متن کاملOne-step Modification and Structuring of Pdms Surfaces and Its Application in the Bench-top Fabrication of Self-driven Microfluidic Channels
This work describes a one-step method for the chemical modification of polydimethylsiloxane (PDMS) surfaces. Thin films of ionic and non-ionic surfactants were patterned into an array format, transferred onto silicone pre-polymers, and subsequently immobilized onto the PDMS surfaces during vulcanization. Our results show that while all surfactants tested can render PDMS surfaces hydrophilic thr...
متن کاملDevelopment of a single-step immunoassay microdevice based on a graphene oxide-containing hydrogel possessing fluorescence quenching and size separation functions.
An immunoassay, which is an indispensable analytical method both in biological research and in medical fields was successfully integrated into a "single-step" by developing a microdevice composed of a graphene oxide (GO)-containing hydrogel and a poly (dimethylsiloxane) (PDMS) microchannel array with a polyethylene glycol (PEG) coating containing a fluorescently-labelled antibody. Here we used ...
متن کاملDetection of Single-base Mutation by Affinity Capillary Electrophoresis in a Pdms-glass Hybrid Microdevice
An extremely rapid on-chip separation of DNA from its single-base substituted mutant is described. By using affinity capillary electrophoresis (ACE) in a poly(dimethylsiloxane) (PDMS)-glass hybrid microdevice, we achieved the separation within 5 s.
متن کامل